Leading platform for advanced imaging.

The ECLIPSE Ti2 inverted microscope delivers an unparalleled 25mm field of view (FOV) that revolutionizes the way you see. With this incredible FOV, the Ti2 maximizes the sensor area of large-format CMOS cameras without making compromises, and significantly improves data throughput. The Ti2's exceptionally stable, drift-free platform is designed to meet the demands of super-resolution imaging while its unique hardware-triggering capabilities enhance even the most challenging, high-speed imaging applications. Furthermore, the Ti2's unique, intelligent functions guide users through imaging workflows by gathering data from internal sensors, eliminating the possibility of user errors. In addition, the status of each sensor is automatically recorded during acquisition, providing quality control for imaging experiments and enhancing data reproducibility.

In combination with Nikon's powerful acquisition and analysis software, NIS-Elements, the Ti2 is a total innovation in imaging.

Manual model with imaging capability for laser applications. Intelligent features provide interactive guidance through imaging workflows and automatic microscope status detection.

Groundbreaking FOV

As research trends evolve towards large-scale, systems-level approaches, there is an increasing demand for faster data acquisition and higher throughput capabilities. Development of large-format camera sensors and improvements in the data processing capabilities of PCs have facilitated such research trends. The Ti2, with its unprecedented 25mm field of view, provides the next level of scalability, enabling researchers to truly maximize the utility of large-format detectors and future-proof their core imaging platform as camera technologies continue to develop at a rapid pace.

High-power LED illuminator	Built-in fly-eye lens

A compact epi-fluorescence illuminator designed for large FOV imaging is equipped with a quartz fly-eye lens and provides high transmittance across a broad spectrum, including UV. Large diameter fluorescence filters with hard coatings deliver large FOV images with a high signal-to-noise ratio.

Large FOV epi-fl illuminator	Large diameter fluorescence filter cubes

Large diameter observation optics

The diameter of the observation light path has been enlarged in order to achieve a field number of 25 at the imaging port. The resulting large FOV is capable of capturing approximately double the area of conventional optics, enabling users to gain maximum performance from large-format sensors such as CMOS detectors.

Enlarged tube lens	Imaging port with large 25 field number

Objectives for large FOV imaging

Objectives with superior image flatness ensure high quality images from edge to edge. Utilizing the maximum potential of the OFN25 objective significantly accelerates data collection.

Cameras for large-volume data acquisition

Nikon’s FX-format F-mount cameras Digital Sight 50M and Digital Sight 10 are equipped with CMOS image sensors optimized for research use, originally developed for professional D-SLR cameras. This allows high-speed and high-sensitivity live-cell imaging, enabling the best use to be made of the Ti2’s large FOV.

Unsurpassed Nikon optics

Nikon's high-precision CFI60 infinity optics, designed for use with a variety of sophisticated observation methods, are highly regarded by researchers for their superb optical performance and solid reliability.

Apodized phase contrast

Nikon's unique apodized phase contrast objectives with selective amplitude filters dramatically increase contrast and reduce halo artifacts to provide detailed high-definition images.

Apodizedphase plate is incorporated in APC objectives	BSC-1 cells captured with CFI S Plan Fluor ELWD ADM 40XC objective

DIC (Differential Interference Contrast)

Nikon's highly-regarded DIC optics provide uniformly clear and detailed images with high resolution and contrast throughout the magnification range. DIC prisms are individually tailored for each objective lens to provide the highest-quality DIC images for every sample.

DIC prisms matched to individual objectives are mounted in the nosepiece	DIC and epi-fluorescence images: 25mm FOV image of neurons (DAPI, Alexa Fluor? 488, Rhodamine-Phalloidin), captured with CFI Plan Apochromat Lambda 60XC objective and DS-Qi2 camera Photo courtesy of Josh Rappoport, Nikon Imaging Center, Northwestern Univ.; Sample courtesy of S. Kemal, B. Wang, and R. Vassar, Northwestern Univ.

NAMC (Nikon Advanced Modulation Contrast)

This is a plastic-compatible, high-contrast imaging technique for unstained, transparent samples such as oocytes. NAMC provides pseudo-three-dimensional images with a shadow-cast appearance. The direction of contrast can be easily adjusted for each sample.

NAMC objective lenses contain rotatable modulators	Mouse embryos, captured with CFI S Plan Fluor ELWD NAMC 20XC objective

Epi-fluorescence

The Lambda series objectives, utilizing Nikon's proprietary Nano Crystal Coat technology, are perfect for demanding, low-signal, multi-channel fluorescence imaging that requires high transmission and aberration correction over a wide wavelength range. Combined with new fluorescence filter cubes that offer improved fluorescence detection and stray light countermeasures such as the Noise Terminator, the Lambda series objectives demonstrate their power in weak signal observations such as single-molecule imaging and even luminescence-based applications.

Luminescence image: HeLa cells expressing BRET-based calcium indicator protein, Nano-lantern (Ca2+). Sample courtesy of Prof. Takeharu Nagai, The Institute of Scientific and Industrial Research, Osaka University

Focus perfected

Even the slightest change in temperature and vibrations in the imaging environment can greatly impact focus stability. The Ti2 eliminates focus drift using both static and dynamic measures to enable faithful visualization of the nanoscopicand microscopic world during long time-lapse experiments.

The detector portion of the Perfect Focus System (PFS) has been detached from the nosepiece in order to reduce mechanical load on the objective nosepiece. This new design also minimizes heat transfer, which contributes to a more stable imaging environment. Towards this end, the power consumption of the Z-drive motor has also been reduced. Combined, these mechanical redesigns result in an ultra-stable imaging platform, perfectly suited for single-molecule imaging and super-resolution applications.

High stability Z-focusing mechanism remains adjacent to the nosepiece even in expanded configurations. ① PFS nosepiece ② PFS Measuring Unit

Assist Guide

It is no longer necessary to memorize complex microscope alignment and operation procedures. The Ti2 integrates data from sensors to guide you through these steps, eliminating user error and enabling researchers to concentrate on their data.

Continuous display of microscope status

A collection of built-in sensors detects and relays status information for a variety of components in the microscope. All of the status information is recorded in the metadata when you acquire images with a computer, so you can easily recall acquisition conditions and/or check for configuration errors.

A collection of built-in sensors detects and relays status information for a variety of components in the microscope. All of the status information is recorded in the metadata when you acquire images with a computer, so you can easily recall acquisition conditions and/or check for configuration errors.

Microscope status can be viewed on a tablet and also determined based on status lights on the front of the microscope, enabling status determination in a dark room.

Guidance for operational procedures 

The Ti2's Assist Guide function provides interactive step-by-step guidance for microscope operation. The Assist Guide can be viewed on a tablet or PC, and integrates real time data from built-in sensors and an internal camera. The Assist Guide is designed to help users through alignment procedures for both experiment setup and troubleshooting.

① Move the field diaphragm image to the center of the field view ② Remove the Bertrand lens from the optical path ③ Select an observation port

 Automatically detect errors

The Check Mode allows users to easily confirm, on either a tablet or PC that all the correct microscope components are in place for their chosen observation method. This capability eliminates time and effort normally required for troubleshooting when the desired observation method is not achieved. This functionality is particularly advantageous when multiple users are involved, each with the potential to make unexpected changes to the microscope settings. Custom check procedures can also be pre-programmed.

Intuitive operation

The Ti2 has been completely redesigned, from the overall body design to the selection and placement of every button and switch, for the ultimate in user experience. The controls are easy to use even in the dark, where the majority of imaging experiments are conducted. The Ti2 provides an intuitive and effortless user interface so researchers can focus on the data and not on microscope control.

Thoughtfully designed layout for microscope control

The placement of all of the buttons and switches are based on the type of illumination they control. Buttons that control diascopicobservation are positioned on the left side of the microscope and those that control epi-fluorescence observation are on the right side. Buttons that control common operations are on the front panel. This use of zoning provides an easy-to-remember layout, a desirable feature when operating the microscope in a dark room.

Programmable Function button 

Conveniently located Function buttons allow customization of the user interface. Users can select from more than 100 functions, including control of motorized devices such as shutters and even signal output to external devices via the I/O port for triggered acquisition. Mode functions, which enable instant changing of observation methods by storing the settings of each motorized device, can also be assigned to these buttons.

Intuitive control with joystick and tablet

The Ti2 joystick not only controls stage movement, but the majority of motorized functions on the microscope, including PFS activity. It can display XYZ coordinates and the status of microscope components, providing an effective means for the user to remotely control the microscope. Motorized functions of the Ti2 can also be controlled from a tablet, connected by wireless LAN to the microscope, providing a versatile graphical interface for microscope control.